Gus Assay Protocol
Kimberly Nelson-Vasilchik, Joel Hague, and Albert Kausch, whilst at The Plant Biotechnology Laboratory of the University of Rhode Island, shared their X-Gluc Gus Assay Protocol online.
Beta-Glucuronidase Protocols
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also similar to the LacZ protocols. [Source: Open WetWare]. Open WetWare is a bit like Wikipedia for scientists and researchers working in…
Histochemical GUS assay
Source: Plant Biotechnology Resource & Outreach Center, Michigan State University. They have shared their procedure for the localization of b-glucuronidase in transgrenic tobacco plants using X-Gluc.
Histochemical Localization of GUS Reporter in Plant Tissues
Stanislav Vitha, Microscopy and Imaging Center, Texas A&M University. The protocol is a guideline that should be compatible with many plant specimens. The paper includes a good section of refernces for further reading and alternatve protocls.