Dissecting Conserved cis-regulatory Modules of Glu-1 Promoters
The Crop Journal Volume 7, Issue 1, February 2019, Pages 8-18
The Crop Journal Volume 7, Issue 1, February 2019, Pages 8-18
Use of the β-Glucuronidase (GUS) Reporter System to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1)Authors: Zhe LiangHilde-Gunn Opsahl-Sorteberg Read the full article here and buy here. Abstract: The DEFECTIVE KERNEL1 (DEK1) gene encodes the unique plant calpain protein, which is fundamental for cell specification, development, and growth of land plants. The β-glucuronidase (GUS) reporter gene system has been…
New Phtologist. Authors: Bo Yang Cătălin Voiniciuc Lanbao Fu Sabine Dieluweit Holger Klose Björn Usadel. First published: 13 September 2018.
Kimberly Nelson-Vasilchik, Joel Hague, and Albert Kausch, whilst at The Plant Biotechnology Laboratory of the University of Rhode Island, shared their X-Gluc Gus Assay Protocol online.
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also similar to the LacZ protocols. [Source: Open WetWare]. Open WetWare is a bit like Wikipedia for scientists and researchers working in…
Maksym Myronovskyi, Elisabeth Welle, Viktor Fedorenko, Andriy Luzhetskyy
Source: Plant Biotechnology Resource & Outreach Center, Michigan State University. They have shared their procedure for the localization of b-glucuronidase in transgrenic tobacco plants using X-Gluc.
Part of the Methods in Molecular Biology™ book series (MIMB, volume 49). The β-Glucuronidase (gus) Reporter Gene System
Gillian A. Hull, Martine Devic
Pages 125-141
Stanislav Vitha, Microscopy and Imaging Center, Texas A&M University. The protocol is a guideline that should be compatible with many plant specimens. The paper includes a good section of refernces for further reading and alternatve protocls.
First published in Current Genetics
March 1990, Volume 17, Issue 3, pp 261–264.