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The chromogenic substrate
X-Gluc is used in a variety of applications for the detection of the β-glucuronidase enzyme. Upon
reduction, XGluc produces a localized colour, making it useful in
identifying GUS gene presence in most cell types and for the
detection of the GUS gene fusion marker in plants.
X-Gluc has reported
applications in the detection of contaminated food samples such as meat,
dairy, and shellfish products.
X-Glucuronide also has clinical
applications in the assessment of urinary tract infection by detecting
the presence of E. coli. It has gained international acceptance as an
accurate indicator for the presence of
E. coli in potable water samples by reducing false positives and
negatives found with traditional methods.
BENEFITS
> Saves time compared to other E.
coli detection methods... 24 hour direct plating method
> Minimizes errors...less than 1%
false negatives and less than 5% false positives
> Easy visual detection...λmax
is in visible range
> Sensitive for presence/absence of
E. coli...eliminates limitations of very rapid presence/absence or
chemiluminometric methods
|
Product |
5-Bromo-4-chloro-3-indolyl b-D-glucuronic
acid
cyclohexylammonium salt (xgluc, xglucuronide) |
|
Molecular Formula |
C20H26BrClN2O7 |
|
Molecular Weight |
521.80 |
|
CAS No. |
114162-64-0 |
|
Appearance |
Crystalline white
powder. |
|
Solution |
Clear solution when
dissolved in DMF (5mg/ml). |
|
Purity |
> 98% by TLC
and NMR. |
| -
300 MHz 1H-NMR in D2O |
Conforms with the
structure. |
|
Storage |
Store at 4
° C or below and protect from light and
moisture. |
To buy X-Gluc view our
pricing & order page
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